Plasmid

Part:BBa_K5302015:Design

Designed by: Bojun Yang   Group: iGEM24_USTC   (2024-10-01)


pBBR-AIDA-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal XbaI site found at 3421
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
    Illegal NotI site found at 1057
    Illegal NotI site found at 4303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal BglII site found at 1803
    Illegal BglII site found at 3414
    Illegal BamHI site found at 5429
    Illegal XhoI site found at 4276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal XbaI site found at 3421
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal XbaI site found at 3421
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 6052
    Illegal AgeI site found at 4137
    Illegal AgeI site found at 5892
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

AIDA: Escherichia coli mCherry: Mushroom coral

References